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Northern blots of RNA isolated from L. sakei L45 wt and mutants hybridized with a lasA-specific probe (see Materials and Methods and Table 1). (A) Lanes 1 and 6, L45-2.1 (wild type); lanes 2 to 4, lasX mutants L45-5.3, L45-5.6, and L45-5.7, respectively; lane 5, L45-3.1 (Bac−Imm−). (B) Lane 1, L45-2.1; lane 2, L45-3.1; lane 3, L45-5.1(lasM::IS1163); lane 4, L45-5.2(lasT::IS1163); lane 5, L45-5.7 (lasX::IS1520); lane 6, L45-5.8 (lasN::IS1520).
