TABLE 2.
Number of chloramphenicol-resistant colonies detected following electroporation into different bacterial strains
| Reaction | DNA electroporated (amt, ng)a | Preincubation with MuA | No. of chloramphenicol-resistant colonies (CFU/μg of DNA)
|
|||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|
|
E. coli K-12
|
E. coli (BL21) | S. enterica serovar Typhimurium L2 SL5676 | E. carotovora LMG2404 | Y. enterocolitica serotype O:3 (6471/76-C) | ||||||||
| MC10161 | DH10B | HB101 | ||||||||||
| Standard | Cat-Mu (12) | Yes | 9 × 105 | 2 × 105 | 6 × 104 | 3 × 104 | 3 × 104 | 1 × 105 | 2 × 104 | |||
| Cat-Mu (12) | No | —c | — | — | — | — | — | — | ||||
| Control | Cat-Mu (500) | No | 3 × 101 | 2 × 101 | 3 × 101 | 2 × 101 | — | — | — | |||
| pBR322-Cmb (1) | No | 2 × 109 | 3 × 108 | 3 × 108 | 4 × 108 | NDd | ND | ND | ||||
a
One microliter of DNA solution was electroporated. For standard reactions, the standard assembly reaction solution was diluted 1:4 with water. For control reactions, the DNA was in solution in water.
b
Electroporation of plasmid pBR322-Cm DNA served as a control for competence status.
c
—, no colonies detected.
d
ND, not determined.