TABLE 3.

Transposon integration sites and target site duplications

Strain	Clone	Sequencea	Transposon orientationb	Genetic locationc
				Gene	Coordinates	Section
E. coli K-12	C1	tttcaatataTTGCT(Cat-Mu)TTGCTggagtttgag	+	mrdA	5173-5177	58
DH10B	C2	ggcgacacctACGGA(Cat-Mu)ACGGAcgcgttttta	+	yhdX	10168-10172	295
	C4	tgacgatgccGTTGC(Cat-Mu)GTTGCggtagcaccg	+	hrpB	2039-2043	14
	C5	ccaggtcataTTCAG(Cat-Mu)TTCAGgccatcatct	−	bglX	6810-6814	192
	C6	gtcgctaatgCCGGA(Cat-Mu)CCGGAgacaatacca	−	ycjJ	10126-10130	117
	C7	tcactccagcGCAGC(Cat-Mu)GCAGCaccatcaccg	−	lacZ	8143-8147	31
	C9	caaagtatgcCCGTC(Cat-Mu)CCGTCtggccagtgc	−	fes	10020-10024	53
	K2	tgtttaattgCCGGA(Kan-Mu)CCGGAtgtcagacat	+	yhjG	1748-1752	319
	K3	aggtgataccCTGGC(Kan-Mu)CTGGCggcctgcctg	−	yrfI	7397-7401	305
S. enterica	S5	tcaacatcaaGCGGC(Cat-Mu)GCGGCaggaaagagg	+	Unknown
serovar	S6	cgaca-caacAGCAA(Cat-Mu)AGCAAcctggtacag	+	Unknown
Typhimurium	S7	gctc-t-acgCAGAC(Cat-Mu)CAGACgatgtaacgt	+	Unknown
LT2 SL5676	S8	gtattgcagcGCAGG(Cat-Mu)GCAGGcgctggtgaa	+	Unknown
E. carotovora	E1	gagctccggcGTAGG(Cat-Mu)GTAGGcgagtccacc	+	Unknown
type strain	E2	ctgcgtgctgCTGCC(Cat-Mu)CTGCCgattctgttt	+	Unknown
LMG2402	E3	atcgatcacgCTATC(Cat-Mu)CTATCgataaagcta	+	Unknown
	E4	attcgt-tctGTCTG(Cat-Mu)GTCTGggttcaccaa	+	Unknown
Y. enterocolitica	Y9	cggca-tattTTGGG(Cat-Mu)TTGGGggctaaattt	+	Unknown
serotype	Y10	aaatgagtatCCGGC(Cat-Mu)CCGGCatctgaatat	+	Unknown
O:3 (6471/76-c)	Y11	acggatactgGCAGG(Cat-Mu)GCAGGtaaagaaatc	+	Unknown
	Y12	gcg-aattatGCCGC(Cat-Mu)GCCGCtgcatcagtg	+	Unknown

^aNucleotides that were difficult to interpret in the sequencing analysis are indicated by hyphens. Target site duplications are in boldface capital letters.

^bCompared to the genomic sequences shown, the transcription from the transposon proceeds from left to right (+) or from right to left (−).

^cGenetic locations of E. coli insertions were determined by comparison to the K-12 strain MG1655 complete genome (GenBank accession numbers AE000111 to AE000510).