FIG. 2.

Effect of tetracycline treatment on in vitro wRi Wolbachia infection. The numbers indicate the cell culture passages following the initiation of tetracycline treatment. In this experiment, the ability to detect Wolbachia via PCR amplification was lost prior to the ninth passage. The ability to detect Wolbachia via PCR amplification persisted beyond 30 passages with in vitro infections that had not been treated with tetracycline. A molecular size standard (Std) is shown in the first lane (123 Ladder; Gibco). The arrow indicates 492 bp.