FIG. 6.

Fed-batch-based two-liquid-phase biotransformation with E. coli JM101(pSPZ3) at a phase ratio of 0.5. The second organic phase consisted of BEHP as the organic carrier solvent, 2% (vol/vol) pseudocumene, and 1% (vol/vol) n-octane (as the inducer). Addition of the organic phase occurred 1 h after feed initiation (arrow). Experimental details of the fed batch are described in Materials and Methods. (A) Reactant and octane concentrations during the fed-batch experiment. All concentrations represent the sum of the respective concentrations in the organic and aqueous phases. (B) Formation of E. coli JM101(pSPZ3) biomass and development of the specific product formation rate, which was calculated by determining the rate of total product formation per gram of CDW as the average for an interval between two data points.