TABLE 1.
Apparenta Vmax and Ks values of E. coli JM101(pSPZ3) for different substrates
| Substrate | Apparent Vmax [U (g of CDW)−1] | Apparent Ks (μM) | Vmax/Ks [U μM−1 (g of CDW)−1] |
|---|---|---|---|
| Pseudocumene | 351 ± 8 | 202 ± 8 | 1.7 |
| 3, 4-Dimethylbenzyl alcohol | 93 ± 3 | 24 ± 4 | 3.9 |
| 3, 4-Dimethylbenz-aldehydeb | ≥560 | ≥ 8,000 | NDc |
| Toluene | 134 ± 9 | 87 ± 17 | 1.5 |
| Benzyl alcohol | 225 ± 9 | 85 ± 4 | 2.6 |
| Benzaldehydeb | ≥18 | ≥ 20,000 | ND |
a
The term apparent refers to the fact that these kinetic values were determined for whole cells. Values are means ± standard errors.
b
The kinetic values for benzaldehydes could not be determined accurately because relevant substrate concentrations were in a range at which measurements were hampered by toxicity and limited solubility of the substrates.
c
ND, not determined.