FIG. 1.
13C-NMR spectra of ethanolic cell extracts. A B. pasteurii strain (DSM 33T) was grown in SMM (2% urea) (A), in SMM (2% urea) with 0.5 M NaCl (B), in SMM (2% urea) with 1.0 M NaCl (C), or in SMM (2% urea) with 1.5 M NaCl (D). Resonances originating from glutamate (g), ectoine (e), and the standard [D4-3-(trimethylsilyl)propionate] (∗) are indicated. Chemical shifts [given in parts per million relative to the standard D4-3-(trimethylsilyl)propionate] for glutamate are 27.9, 34.6, 55.8, 175.3, and 184.1 and for ectoine are 19.6, 23.5, 38.3, 54.4, 161.9, and 177.8, respectively.