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. 2002 Feb;68(2):772–783. doi: 10.1128/AEM.68.2.772-783.2002

FIG. 4.

FIG. 4.

Ectoine biosynthetic pathway and Northern blot analysis of the B. pasteurii ectABC region. (A) Ectoine biosynthetic pathway from the precursor l-aspartate-β-semialdehyde. (B) Genetic organization of the ectABC genes in B. pasteurii and position of the RNA probe used for the transcriptional analysis of the ectABC gene cluster. (C) Northern blot analysis of the ectABC operon. Total RNA was isolated from B. pasteurii strain DSM 33T grown in SMM (2% urea) with or without 0.5 M NaCl and hybridized to an ectB-specific RNA probe.