TABLE 2.
Osmotically induced ectoine biosynthesis in B. pasteurii (DSM 33T)
| Mediuma | Ectoine (mmol per g [dry weight])b |
|---|---|
| SMM (680 mosmol per kg of water) | NDc |
| SMM with 0.4 M NaCl (1,400 mosmol per kg of water) | 0.40 ± 0.020 |
| SMM with 0.4 M KCl (1,400 mosmol per kg of water) | 0.59 ± 0.034 |
| SMM with 0.68 M sucrose (1,400 mosmol per kg of water) | 0.59 ± 0.070 |
| SMM with 0.68 M lactose (1,400 mosmol per kg of water) | 0.36 ± 0.067 |
| SMM with 1.1 M glycerol (1,400 mosmol per kg of water) | ND |
| SMM with 0.4 M NaCl + 1 mM glycine betaine (1,400 mosmol per kg of water) | ND |
| SMM with 0.68 M sucrose + 1 mM glycine betaine (1,400 mosmol per kg of water) | ND |
a
Cultures of B. pasteurii (DSM 33T) were grown in SMM (2% urea) with the indicated ionic and nonionic osmolytes. Ectoine was extracted from the freeze-dried cell pellet and was measured by HPLC analysis.
b
The data shown are means of two independently grown cultures, and for each of these cultures the ectoine content was determined twice.
c
ND, no ectoine was detectable.