TABLE 1.
Excitation wavelengths for single-photon laser scanning microscopy and 2P-LSM for various nucleic acid-specific fluorochromes for staining of bacteria in biofilms
| Fluorochrome | Excitation wavelength(s) (nm)
|
|
|---|---|---|
| Single photona | Two photon | |
| DAPI | 358 (360) | 780-800 |
| Syto40 | 420 (442) | 830-860 |
| SytoxBlue | 436 (442) | 840-870 |
| Syto9 | 480 (488) | 860-890 |
| Syto13 | 481 (488) | 860-890 |
| SybrGreen | 497 (488) | 790, 870-900 |
| SybrGold | 495 (488) | 760, 850-890 |
| SytoxGreen | 500 (488) | 780, 880-900 |
| AO | 500 (488) | 780-900 |
a
The values for single-photon excitation are the optimal wavelengths; the values in parentheses are the usual wavelengths of lasers that are available.