(A) Comparison of the pilD locus in different gram-negative proteobacteria and nonproteobacteria. orf2073 encodes a thermostable carboxypeptidase and orf2071 encodes a hypothetical protein in Thermus. ssr1853 encodes a hypothetical Synechocystis protein, and sll1059 encodes an adenylate cyclase. DR2065 is a pilD homologue in Deinococcus. The Deinococcus ORFs DR2063, DR2064, and DR2066 encode a polynucleotide phosphorylase, a hypothetical 17.3-kDa protein, and a conserved hypothetical protein, respectively. comC is a pilD homologue in B. subtilis. spoIIB and folC encode a sporulation factor and the enzyme folypoly-γ-glutamate synthetase-dihydrofolate synthetase, respectively. (B) Comparison of the genetic organization of the pilQ cluster in different gram-negative proteobacteria and nonproteobacteria. The Thermus ORFs orf559, orf566, and orf567 encode isopropylmalate dehydrogenase, chorismate synthase, and shikimate kinase, respectively. DR0775 is an aroC homologue and DR0776 is an aroK homologue in Deinococcus. slr1273 and sll1174 are predicted to encode hypothetical proteins in Synechocystis.