FIG. 1.

PCR amplification of P. piscicida mitochondrial cytochrome b and its 5" flanking region and organization of the gene fragments. (A) PCR amplification with primers PPCOX3 and PPCOB (Table 2). (B) Organization of the three PCR-amplified gene fragments. cox3, cytochrome oxidase subunit 3; cob, Ppcob; X, sequence that does not exhibit similarity to known genes in DNA databases. Coding regions are indicated by solid boxes. The dashed lines in the X region in PpcytbG2 and PpcytbG3 indicate deleted regions. The arrow indicates the region used to design P. piscicida-specific primers.