FIG. 1.

(A) Temporal appearance of conidium-bearing tips in cultures of P. cyclopium. The percentage of conidiating tips is expressed as the conidiation percent. Assays were carried out in shaken flask cultures inoculated with 10⁵ conidia ml⁻¹. The points of 50% conidiation, taken as the reference point at which conidiation was considered to have been reached, are indicated by dotted lines. The occurrence of germination (small arrow), conidiation in F medium (hollow arrowhead) and mF medium (filled arrowhead), and conidiation induction in F medium (hollow arrow) and mF medium (filled arrow) are indicated in the figure. (B) Effect of the inoculum load on conidiation time in cultures of P. cyclopium. Assays were carried out in shaken flasks with cultures inoculated with increasing inoculum loads. In both figures, triangles represent growth in fresh F medium and circles represent growth in mF medium. The mF medium was prepared by growing the fungus in a 10-liter fermenter for 36 h with an inoculum of 10⁶ conidia ml⁻¹. Values are expressed as the means of three independent experiments. Standard deviations (not shown) are lower than ±10%.