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Suppression of fig2Δ/fig2Δ zygote phenotypes by increased osmolarity acts specifically on morphogenetic and not cell-cell fusion defects. DIC and FM4-64-stained fluorescence images of zygotes formed in matings carried out in YPAD-1.0 M sorbitol medium. Micrographs are of A) YSE20 × YSE21 (WT) (A) and (panels B, C, and D) YSE29 × YSE30 (fig2Δ) zygotes display sorbitol-suppressed, normal conjugation bridge morphogenesis, but remain defective in cell fusion.
