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. 2002 Oct;1(5):811–822. doi: 10.1128/EC.1.5.811-822.2002

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Copyright © 2002, American Society for Microbiology

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FIG. 3. — Absence of FIG2 results in increased inviability under mating conditions. A) Viability in the presence of α-factor. MATa WT (YSE20 and YSE430) and fig2Δ (YSE29 and YSE548) strains were treated with pheromone for the indicated times and assayed for viability by methylene blue exclusion. (B) Mating cells from bilateral crosses of Wt (YSE21 × YSE22) and fig2Δ (YSE29 × YSE30) strains were assayed for viability by methylene blue exclusion immediately following mating for 4 h on solid media under the conditions indicated. Values represent percentages from over 800 cells counted. (C) Zygote formation rates for the crosses indicated in panel B. (D) Viability of zygotes in mating mixtures shown in panel B.