FIG. 1.

Deletion of the PAF1 gene causes a decrease in mRNA levels for many cell cycle-regulated genes. Asynchronous cultures of wild type (YJJ662) and paf1Δ (YJJ664) cells were grown to a Klett density of 60 (2 × 10⁶ cells/ml), total RNA was harvested, and 10 μg of RNA per lane was fractionated and used to detect mRNAs for specific cell cycle-regulated genes as described in Materials and Methods. The cycle peak designations are from the microarray analysis of Spellman et al. (45; http://genome-www.stanford.edu/cellcycle/). The fold decrease of each mRNA in the paf1Δ strain compared to that in the wild type was calculated from an average of at least two experiments after normalization to 18S rRNA. RPS9A is an example of a gene that decreases in paf1Δ but is not cell cycle regulated. PIR3 is an example of a cell cycle-regulated gene that does not decrease in a paf1Δ strain. Results for two different strain backgrounds are shown for PIR3. The other mRNAs shown in this figure were from the D273-10b background. Similar results were obtained from both strains for the other mRNAs shown in this figure.