FIG. 3.

mRNA abundance for cycling genes is decreased throughout the cell cycle in the paf1Δ strain. Fractionated total RNA from synchronized wild-type (YJJ755) and paf1Δ (YJJ756) cells was analyzed with probes (see Table 2) that detect periodic genes that peak in the indicated parts of the cell cycle. PIR3 expression peaks in M/G₁ but is not affected by lack of Paf1. Each graph shows the normalized amount of mRNA for that gene over a period of 1.5 doublings for wild-type and paf1Δ strains. The mRNA/18S rRNA units are arbitrary phosphorimager numbers and are not comparable between probes. However, each individual panel represents equal amounts of RNA from wild-type and paf1Δ cells fractionated on the same gel for direct comparison.