FIG. 5.

Gene expression profiles of swi4Δ and mbp1Δ strains are not identical to that of a paf1Δ strain. Asynchronous cultures of wild type (YJJ662), paf1Δ (YJJ664), swi4Δ (YJJ1000), and mbp1Δ (YJJ1067) cells were grown to a Klett density of 60 (2 × 10⁶ cells/ml). Total RNA was harvested, 10 μg of RNA per lane was fractionated, and specific mRNAs were detected using probes for cell cycle-regulated genes and normalized to 18S rRNA. The fold change of each mRNA in paf1Δ, swi4Δ, and mbp1Δ strains compared to that for the wild type is an average of at least two experiments. PIR3 is a cell cycle-regulated gene that does not decrease in a paf1Δ strain (see Fig. 1). Note that the asynchronous samples shown in this figure were from the D273-10b genetic background; similar results were obtained in the A364a background.