FIG. 8.

Both Mbp1 and Swi4, when overexpressed, can partially suppress paf1Δ. Spot assays were performed as described in Materials and Methods. WT + v1, YJJ662 transformed with YEp24; paf1Δ + v1, YJJ664 transformed with YEp24; paf1Δ + Mbp1, YJJ664 transformed with BK72; paf1Δ + v2, YJJ664 transformed with YEp352; paf1Δ + Swi4, YJJ664 transformed with B327. Spots from left to right were made by applying 4 μl of a cell suspension containing 10⁷, 10⁶, 10⁵, 10⁴, and 10³ cells/ml. Similar results were obtained with two independent sets of transformants.