TABLE 2.
Oligos used for strain construction and as probes
| Oligo | Use | Sequence |
|---|---|---|
| 230 | swi4Δ verification | 5′-GTTCAAGGTGGGTATGGTAGG-3′ |
| 231 | swi4Δ verification | 5′-TATGCGTTTGCCCTCAAATCC-3′ |
| 287 | swi4Δ strain Kanr replacement | 5′-CCTTCTGTCCCTCTTGCGTAGTTCTAAAAGGTTGATTTATTCGAGAAATCCGTACGCT GCAGGTCGAC-3′ |
| 288 | swi4Δ strain Kanr replacement | 5′-AAAAACTCTGATAATATAGTAAAAATTATTGGTACATTGTGATTAAAATATCGATGA ATTCGAGCTCG-3′ |
| 278 | swi4Δ::Kanr verification | 5′-TCAACAATAATTGCTCTTTGCCG-3′ |
| 331 | swi4Δ::Kanr verification | 5′-GCAACTCAAGCGCAATGAGA-3′ |
| 240 | swi4Δ::Kanr verification | 5′-CTCATCTGTAACATCATTGGCAAC-3′ |
| 380 | mbp1Δ strain Kanr replacement | 5′-CTTAACATTCCGAGACACAACGTAAATCCCAGAAACACAAGCATGCGTACGCTGCAGGTCGAC-3′ |
| 381 | mbp1Δ strain Kanr replacement | 5′-CAGTATATGGATACATGTAAAGTTCCTCTATTTATGTATATTTTAATCGATGAATTCG AGCTCG-3′ |
| 382 | mbp1Δ::Kanr verification | 5′-TCAAGTGCGATTGGTCTGCA-3′ |
| 383 | mbp1Δ::Kanr verification | 5′-GCACTGCTTACTGTTATGTC-3′ |
| 282 | SPC25 probe, 5′ primer | 5′-GCCAGCATAGACGCATTTTCGG-3′ |
| 271 | SPC25 probe, 3′ primer | 5′-TGAATCATCGCCGAATACGAAACGTA-3′ |
| 283 | SAS3 probe, 5′ primer | 5′-GTCATTAACAGCAAACGACGAATCGC-3′ |
| 269 | SAS3 probe, 3′ primer | 5′-CCAGTATCGTTTCTCCTAGCTGCATG-3′ |
| 281 | STP4 probe, 5′ primer | 5′-TGGTATCATCATCTTTTGCATCAAGC-3′ |
| 272 | STP4 probe, 3′ primer | 5′-GGATACACCTCCATATCCAAATTGATC-3′ |
| 284 | PCL7 probe, 5′ primer | 5′-ATGGAGCTAAGTTCACCATCAAAAAAAAC-3′ |
| 273 | PCL7 probe, 3′ primer | 5′-CACTCAAGAACTTCGTGCAAATTGTG-3′ |
| 374 | HO probe, 5′ primer | 5′-CGCAAACGTCACGGCTAAC-3′ |
| 375 | HO probe, 3′ primer | 5′-TCAAACTGTAAGATTCCGCCAC-3′ |
| 84 | 18S rRNA probe | 5′-GCTTATACTTAGACATGCAT-3′ |
| 211 | RNR1 probe | 5′-ACACATTTCACAAGCTTCTGGG-3′ |
| 213 | FAR1 probe | 5′-TTCTATCAAACTAGAATGCGGGTGTT-3′ |
| 214 | RPS9A probe | 5′-CCGCCTTCCTTGCAGCATTTCTTCTA-3′ |
| 216 | CYB5 probe | 5′-CCACTACCTTTACTTTGGTTTTCAGA-3′ |
| 217 | FKS1 probe | 5′-CCAATGAATCTCCAATGTGTTTGTGG-3′ |
| 265 | HTB1 probe | 5′-CAGTGGAAGTGGAAGTCTTTTTAGCG-3′ |
| 268 | PIR3 probe | 5′-GAGCCAATTCTACCCTTCCTATCGGT-3′ |
| 344 | CLN1 promoter, 5′ | 5′-CGAAAACACCGCGCGTAAAG-3′ |
| 396 | swi6Δ strain Kanr replacement, 5′ | 5′-AAAAAGAATAATAAAGGGGAACACAGTATAATTCTCGAGAGGCGTACGCTGCAGG TCGAC-3′ |
| 397 | swi6Δ strain Kanr replacement, 3′ | 5′-AGTGCCTATATATCCCATCTGAGTACGTAAAAATTTTGTAAATCGATGAATTCGAG CTCG-3′ |
| 410 | swi6Δ::Kanr verification, 5′ | 5′-GGACCGTTTGGATAAGATCATTCACC-3′ |
| 411 | swi6Δ::Kanr verification, 3′ | 5′-GGTGGAAGGACAGGCTTAAGATCTTGTAG-3′ |
| 588 | CLN1 promoter, 3′ | 5′-CCAACTCGAGTCTAGAAGTGGAGTGGTGGTAGTGC-3′ |
| 597 | FKS1 promoter, 5′ | 5′-AAGGTACCTCTAGAATGTACGGGCAATCAGAATCTG-3′ |
| 598 | FKS1 promoter, 3′ | 5′-AAGGTACCGGTCTGACCGTTGTATGAAAGAC-3′ |
| 599 | GLK1 promoter, 5′ | 5′-AAGGTACCTCTAGAGCTTTAACGTATTCAGGAGCCATG-3′ |
| 600 | GLK1 promoter, 3′ | 5′-AAGGTACCGTATTAGTGGTGGTGTTGTGGTTTAC-3′ |