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. 2002 Oct;1(5):736–757. doi: 10.1128/EC.1.5.736-757.2002

FIG. 10.

FIG. 10.

Chlamydomonas Atx1 rescued S. cerevisiae atx1 and sod1 mutants. (A) Complementation of iron-deficient growth in S. cerevisiae atx1Δ mutant strain SL215. S. cerevisiae strains YPH250 (wt), SL215 (atxΔ), and SL215 transformed with CrAtx1, ScATX1, or control vector pFL61 were serially diluted and grown on SD complete medium containing the iron chelator ferrozine (1.5 mM) in the absence (−Fe) or presence (+Fe) of 350 μM ferrous ammonium sulfate. (B) Complementation of aerobic lysine and methionine auxotrophy of S. cerevisiae sod1Δ mutant. wt, sod1Δ mutant, and sod1Δ strains transformed with CrAtx1, ScATX1, or control vector pFL61 were grown on YPD or SD complete medium lacking lysine or methionine.