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. 2002 Oct;1(5):663–672. doi: 10.1128/EC.1.5.663-672.2002

FIG. 3.

FIG. 3.

Localization of C-terminal-truncated Gcn4p fused to GFP in yeast. S. cerevisiae cells (RH1408, gcn4-103) expressing different GFP-Gcn4p derivatives from the induced MET25 promoter were grown to early log phase in selective medium at 30°C and analyzed by DIC microscopy (right column) and fluorescence microscopy (left column, GFP; middle column, DAPI). Localization of GFP-Gcn4p derivatives lacking either the leucine-zipper amino acid residues 250 to 281 (pME2127), the leucine zipper and the DNA-binding domain from position 221 to 281 (pME2128), or the 112 C-terminal amino acids of Gcn4p (pME2129) are shown. Fusing back NLS2 from position 215 to 249 to the N terminus of truncated Gcn4p (pME2129) enabled the protein to enter the nucleus again (pME2317). The N-terminal amino acids of Gcn4p fused to GFP are indicated.