FIG. 4.

NLS motifs of Gcn4p target the cytoplasmic Aro7 protein to the nucleus. Yeast cells (RH1347) expressing different GFP-Aro7p constructs from the induced MET25 promoter were grown to early log phase in selective medium at 30°C and analyzed by DIC microscopy (right column) and fluorescence microscopy (left column, GFP; middle column, DAPI). Localization of GFP-Aro7p (pME2130) was compared with that of GFP-Aro7p fused with either Gcn4p amino acids 167 to 200 (pME2131), 221 to 281 (pME2132), or Gcn4p amino acids 231 to 249 (pME2133). Bars illustrate the different GFP constructs, and the fused Gcn4p amino acids are indicated.