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A yrb1-51 mutation impairs Gcn4p import into the yeast nucleus. Temperature-sensitive yrb1-51 mutant cells (RH2663) expressing either Aro7p-Gcn4p/NLS-GFP (pME2133) or nontruncated Gcn4p-GFP (pME2126) were grown to early log phase in selective medium at the permissive temperature of 23°C and then shifted to the restrictive temperature of 37°C. Samples were collected at the indicated time points after shifting and analyzed by DIC microscopy and fluorescence microscopy.
