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. 2002 Oct;1(5):704–718. doi: 10.1128/EC.1.5.704-718.2002

FIG. 7.

FIG. 7.

The MATα mating-type locus is conserved in the population. Using one primer combination, overlapping fragments (3 to 10 kb) spanning part of the mating-type locus were PCR amplified from (lanes from left to right in panel 1) the control strain JEC21 and the unrelated serotype D clinical isolates CDC92-18, CDC92-27, and MMRL760. Fragments of identical sizes were amplified from all four strains with nine primer combinations (panels 1 to 4 and 6 to 10). For descriptions of the 10 primer pairs, which are also represented by numbered bars indicating their positions on the MAT locus, see Table 1. One primer pair yielded a larger PCR product for strain MMRL760 (panel 5, lane 4), indicative of an ∼4-kb insertion between the STE11α and MFα1 genes. DNA sequence analysis revealed that the insertion of a novel mariner-related transposable element resulted in the addition of 4,006 bp relative to the JEC21 serotype D α allele and the creation of a TA target site duplication.