TABLE 2.
Phenotypic characterization and phylogenetic affiliations of the PHBV-degrading denitrifying isolates
| Phylogenetic group and strain | Source | Denitrification rate with PHBV (NO3−-N removed h−1 g[dry wt])−1a | Major quinone type | 16S rDNA sequence comparison
|
|||
|---|---|---|---|---|---|---|---|
| Length of sequence determined (bases) | Species as closest relative | Accession no. | Similarity (%) | ||||
| β-Proteobacteria | |||||||
| NOS3 | NS1 | ND | Q-8 | 1,526 | [Aquaspirillum] psychrophilum | AF078755 | 97.6 |
| NOS8 | NS1 | ND | Q-8 | 1,526 | [Aquaspirillum] psychrophilum | AF078755 | 97.7 |
| NA10Bb | NS1 | 19 | Q-8 | 1,521 | Acidovorax avenae subsp. citrulli | AF078761 | 96.9 |
| NSP4 | NS2 | 12 | Q-8 | 1,521 | Comamonas terrigena | AF078766 | 96.1 |
| NSP5 | NS2 | 12 | Q-8 | 1,521 | C. terrigena | AF078772 | 96.7 |
| NSP7 | NS2 | 13 | Q-8 | 1,521 | C. terrigena | AF078772 | 96.1 |
| NSP8 | NS2 | 14 | Q-8 | 1,521 | C. terrigena | AF078772 | 96.7 |
| NS20-1b,c | NS2 | ND | Q-8 | 695 | A. avenae subsp. citrulli | AF078761 | 96.0 |
| NS20-2c | NS2 | ND | Q-8 | 696 | Acidovorax defluvii | Y18616 | 97.1 |
| KSP1 | OS1 | 18 | Q-8 | 1,521 | A. avenae subsp. citrulli | AF078761 | 98.3 |
| KSP2 | OS1 | 16 | Q-8 | 1,521 | A. avenae subsp. citrulli | AF078761 | 98.7 |
| KSP3b | OS1 | 19 | Q-8 | 1,522 | A. avenae subsp. citrulli | AF078761 | 96.9 |
| KSP4b | OS1 | 19 | Q-8 | 1,523 | A. avenae subsp. citrulli | AF078761 | 96.9 |
| OS-3 | OS2 | ND | Q-8 | 1,495 | C. terrigena | AF078772 | 96.1 |
| OS-6 | OS2 | ND | Q-8 | 1,522 | Acidovorax temperans | AF078766 | 97.5 |
| OS-9 | OS2 | ND | Q-8 | 1,523 | A. temperans | AF078766 | 96.6 |
| OS-14 | OS2 | ND | Q-8 | 1,521 | C. terrigena | AF078772 | 96.7 |
| OS-19 | OS2 | ND | Q-8 | 1,523 | A. avenae subsp. citrulli | AF078761 | 97.2 |
| TS-18 | TS | ND | Q-8 | 1,526 | [Aquaspirillum] psychrophilum | AF078755 | 97.6 |
| TS20-3b,c | TS | ND | Q-8 | 697 | A. avenae subsp. citrulli | AF078761 | 95.8 |
| γ-Proteobacteria | |||||||
| P400Y-1 | NS2 | 10 | Q-9 | 1,498 | Pseudomonas citronellolis | Z76659 | 98.0 |
| PG3-3 | OS1 | ND | Q-8 + MK-8 + DMK-8 | 1,505 | Aeromonas hydrophila | X60404 | 99.7 |
| PG4-1 | OS2 | ND | Q-8 + MK-8 + DMK-8 | 1,505 | A. hydrophila | X60404 | 99.7 |
a
ND, not determined.
b
These strains are more closely related to an unidentified bacterium, strain LW1 (99.9% similarity) (Fig. 4).
c
Strains isolated at 20 to 22°C. All other strains were isolated at 28 to 30°C.