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. 2025 Apr 12;53(6):2267–2276. doi: 10.1007/s15010-025-02538-4

Table 1.

Examples of microbiome-sparing antimicrobial agents

Name Mechanism of Action Pathogens Targeted Advantages Disadvantages
Lolamicin Inhibits LolCDE complex, essential for lipoprotein transport in Gram-negative bacteria Gram-negative bacteria (E. coli, K. pneumoniae, E. cloacae) Preserves microbiota, novel mechanism Limited to Gram-negative pathogens, not effective against P. aeruginosa or A. baumannii
SMT-738 Targets LolC/E complex in Enterobacteriaceae Gram-negative pathogens (Enterobacterales) Microbiome-sparing, selective targeting Early-stage development, limited clinical data; mainly selective for Enterobacterales
Afabicin Inhibits FabI, an enzyme in fatty acid biosynthesis S. aureus (Gram-positive bacteria) Microbiome-sparing, targets Gram-positive pathogens Narrow-spectrum, only effective against Gram-positive bacteria
Debio 1453 Inhibits FabI, disrupting fatty acid biosynthesis N. gonorrhoeae Microbiome-sparing, rapid bactericidal activity Limited data, in development stage
FP-100 (Hygromycin A) Inhibits ribosomes of spirochetes B. burgdorferi, Treponema pallidum, Fusobacterium nucleatum Selectively targets periodontal pathogens without disrupting beneficial bacteria (Streptococcus parasanguinis, Bifidobacterium longum) Still under development, limited data
FtsZ Inhibitors (e.g. TXA707, TXA709) Inhibit polymerization of the FtsZ protein, disrupting bacterial cell division Drug-resistant Gram-positive bacteria (MRSA, VRSA, DNSSA) Synergistic with β-lactams, microbiome-sparing Limited to Gram-positive pathogens, limited data
Ribaxamase β-lactamase that targets excess IV β-lactam antibiotics in the GI tract Prevents damage from broad-spectrum antibiotics in gut flora Reduces dysbiosis, prevents antibiotic resistance and C. difficile infection Limited to co-administration with IV β-lactams, early trial phase
Fidaxomicin Inhibits bacterial RNA polymerase, disrupting transcription C. difficile Targets C. difficile, microbiome-sparing Narrow-spectrum, limited to C. difficile infections
Ibezapolstat Targets DNA polymerase IIIC (Pol IIIC) C. difficile 100% clinical cure rate in trials, microbiome-sparing Early-stage trials, high cost
Ridinilazole Binds to minor groove of DNA, inhibiting transcription C. difficile Targets C. difficile, microbiome-sparing Limited to C. difficile infections, i.e. narrow-spectrum