FIG. 3.
TLC of ɛ-PL produced by K. kifunense strain MN-1. (A) Lanes: 1, l-lysine (1 μg); 2 and 3, heat-treated polymers in the presence of 1 or 6 N HCl, respectively; 4 and 5, heat-treated blank agar pieces (1 or 6 N HCl). (B) Hydrolysate of DNP-protected polymer. Lanes: 1, l-lysine (1 μg); 2, N,N′-di(2, 4-DNP)-l-lysine (10 μg); 3, Nα-2,4-DNP-l-lysine (10 μg); 4, hydrolysate of DNP-protected polymer produced by MN-1; 5, Nɛ-2,4-DNP-l-lysine (10 μg).