TABLE 2.

Changes in act and pfl mRNA levels, PFL-AE activity, and formate-to-lactate ratio during growth of S. bovis in batch culture

Time (h)	Growth ratea	Relative amt of mRNAb		Relative PFL-AE activityc	Formate-to-lactate ratiod
		act	pfl
2	0.92	1.0	1.0	100	0.16
5	0.31	1.4	1.8	150	0.29
6	0.14	2.4	3.4	220	0.51

^aGrowth rate is expressed as the increase in the amount of cellular nitrogen (in milligrams) per hour.

^bRelative amounts of act and pfl mRNA as estimated by Northern blot analysis.

^cPFL-AE activity was estimated by cleavage of the PFL protein.

^dRatio of the amount of formate to the amount of lactate produced in 1 h.