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Normalized Td (temperature of dissociation) curves determined by using transcribed RNA (338 to 927, E. coli numbering) of target 16S rDNA fragments and native RNA of nontarget microorganisms. Numbers in parentheses indicate the number of mismatches between probe and target sequences. All curves shown are the result of duplicate experiments. Only target RNA was used for probe S-∗-Edn-0656-a-A-18.
