FIG. 4.

DNA microarray temperature-of-dissociation study for transcribed RNA fragments derived from DGGE bands on DNA microarrays. Numbers in parentheses indicate the number of mismatches between probe and target sequences. All elution curves represent the means of four replicate experiments on one chip. Hybridization to RNA containing more than three mismatches could not be detected at the beginning of the melting curve except for the duplex of S-∗-Edsrb-0656-a-A-19 and T-1. A washing temperature of 46°C (vertical line) was used to achieve suitable mismatch discrimination.