FIG. 5.

Hybridization of lissamine rhodamine-labeled RNA to DNA microarray. RNAs (338 to 927, E. coli numbering) were transcribed from PCR-amplified 16S rDNA fragments from the DGGE gel, fragmented with magnesium, and labeled with lissamine rhodamine. Probes, their locations, and the number of mismatches between each probe and the RNA are shown in Table 2. The DNA microarray was hybridized to in vitro-transcribed 16S rRNA fragments from bands T-1, E-1, and E-2, shown in panels T-1, E-1, and E-2, respectively.