TABLE 1.
Bacterial strains and plasmids used in this study
| Strain or plasmid | Relevant characteristics | Reference or source |
|---|---|---|
| E. coli | ||
| K-12 | DSM 498 | |
| DH1 | F−endA1 gyrA96 hsdR17 (rK− mK+) recA1 supE thi-1 λ− | 8 |
| DH5α | recA1 endA1 gyrA96 thi-1 hsdR17 supE44 relA1 ΔlacU169 (φ80 lacZΔM15) | 8 |
| JM109 | endA1 recA1 gyrA96 thi hsdR17 (rK− mK+) relA1 supE λ− Δ(lac-proAB) [F′ traΔ36 proAB lacIqZΔM15] | 26 |
| SMH50 | supE44 endA1 hsdR17 gyrA96 thi-1 relA1 Δ(lac-proAB) F′ [traD36 proAB+lacIqlacZΔM15] | 17 |
| TG1 | Δ(lac-pro) supE thiE hsdD5/F′ [traD36 proA+B+ lacIqlacZΔM15] | 6 |
| Top10 | F−araD139 Δ(ara leu)7697 ΔlacX74 galU galK rpsL deoR φ80dlacZΔM15 endA1 nupG recA1 mcrA Δ(mrr hsdRMS mcrBC) | Invitrogen (San Diego, Calif.) |
| Plasmids | ||
| pMa/c5-914 | Apr Cmrc1857ts λ PL/PR, translational initiation region | Personal gift from T. Wagnera |
| pMa/c5-914::cphA | pMa/c5-914 carrying 2.6-kb PCR product from Synechocystis sp. strain PCC6803 genomic DNA harboring cphA | This study |
a
Institut für Molekulare Genetik der Georg-August-Universität, Göttingen, Germany.