TABLE 1.
Bacterial strains and plasmids
| Strain or plasmid | Relevant characteristics | Source or reference |
|---|---|---|
| P. aureofaciens | ||
| 30-84 | Phz+ Rifr wild type | W. W. Bockus |
| 30-84Z | Phz− RifrphzB::lacZ genomic fusion | 20 |
| 30-84.gacA | Phz− Rifr Phz− RifrgacA::Kmr of strain 30-84 | 3 |
| 30-84SGS1 | Phz− Rifr spontaneous gacS mutant of strain 30-84 | This study |
| 30-84SGA2 | Phz− Rifr spontaneous gacA mutant of strain 30-84 | This study |
| 30-84.A2 | Phz− Rifr Kmr spontaneous gacS mutant of strain 30-84 containing Tn5 in an unidentified locus | 3 |
| E. coli | ||
| DH5α | F−recA1 endA1 hsdR17 supE44 ti-1 gyrA96 relA1 Δ(argF-lacZYA) 1169 φ80lacZ ΔM15 | Gibco-BRL |
| HB101 | F−hsdS20 (rB− mB−) supE44 recA1 ara14 proA2 lacY1 galK2 rpsL20 xyl-5 mtl-5 | Gibco-BRL |
| Plasmid | ||
| pLAFR3 | IncP1 Tetr cos+ rlx+ | 25 |
| pRK415 | IncP1 Tetr cos+ rlx+ | |
| pRK2013 | IncP1 tra oriE1 Kmr | 5 |
| pRKgacSB03 | An approximately 3-kb PCR product containing gacS of strain 30-84 cloned into the BamHI site of pRK415 | This study |
| pLSP259 | pLAFR3 containing phzI, phzR, and the phenazine biosynthetic locus on a 20.4-kb EcoRI fragment of strain 30-84 chromosomal DNA | 20 |
| pLSP19-21 | pLAFR3 containing a 23-kb EcoRI fragment of 30-84 genomic DNA that contains the 3′ end of gacS | This study |
| pSTC121 | pLAFR3 containing a 4.5-kb EcoRI-PstI gacA fragment of pSTC110 | 3 |
| pSTC140 | pUC18 containing a 2.2-kb KpnI/PstI gacA fragment of pSTC121 | 3 |