TABLE 2.
Enzyme | Relative activity (%) (mean ± SD) on xylana
|
|
---|---|---|
Soluble | Insoluble | |
XynX′CD | 170 ± 7 | 50 ± 5 |
XynX′TSD-CD | 105 ± 3 | 89 ± 7 |
XynX′CD-TSD | 122 ± 6 | 79 ± 4 |
The activity of each enzyme on unfractionated xylan (1.0% [wt/vol]) was considered to be 100%. Preparations of soluble and insoluble fractions are described in Materials and Methods. Final concentrations of soluble and insoluble substrates were approximately 0.2 and 0.8%, respectively.