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. 2002 Jul;68(7):3651–3654. doi: 10.1128/AEM.68.7.3651-3654.2002

FIG. 2.

FIG. 2.

Effect of incubation temperature on the forms of RSDA expressed from E. coli (pARMH) as shown by Coomassie brilliant blue staining (A) and Western blotting (B). S, cytoplasmic soluble fraction; I, cytoplasmic insoluble fraction. Lanes M, low-molecular-weight standard; lanes S, purified RSDA from the Cytophaga sp.; lanes 1 and 2, cell lysates of E. coli DH5α and E. coli DH5α(pAcUW21), respectively; lanes 3, 5, 7, and 9, soluble fractions of the E. coli DH5α(pARMH) cell lysate at the indicated temperatures (in degrees Celsius); lanes 4, 6, 8, and 10, insoluble fractions of the E. coli (pARMH) cell lysate at the indicated temperatures.