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sgRNA detailed sequence and PAM regions in the following table.
| sgRNA | Sequence (5′-3′) | PAM (5′-3′) |
|---|---|---|
| sgRNA-1 | F:CCAAGCGCTGGGCGTCAAGA R:TCTTGACGCCCAGCGCTTGG |
TGG |
| sgRNA-2 | F:GACTAGTCCATCCAAGACCC R:GGGTCTTGGATGGACTAGTC |
TGG |
| sgRNA-3 | F:AGCCTGTTCTACCACACGTG R:CACGTGTGGTAGAACAGGCT |
GGG |
