TABLE 2.

PCR program in the following table.

Cycle step	Temp	Time	Cycle number
Initial denaturation	95 °C	2 min	1
Denaturation	95 °C	30 s	35
Annealing	58 °C	30 s	35
Extension	72 °C	1 min	35
Final extension	72 °C	10 min	1