TABLE 3.
Anaerobic growth on benzoate, hybridization with the benzoate-CoA ligase (badA) gene, and utilization of 3-chlorobenzoate by the benzoate-degrading genotypes
| Genotype (no. of isolates)a | Speciesb | Growth rate on benzoate (h)c | Hybridization with badAd | Utilization of 3-chlorobenzoatee
|
|
|---|---|---|---|---|---|
| Without benzoate | With benzoate | ||||
| BiesB1 (7) | Rps. palustris | 31.7 (1.2) | + | − | + |
| BiesB5 (2) | Rps. palustris | 16.5 (0.5) | + | − | + |
| HaA5 (2) | Rps. palustris | 18.0 (0.5) | + | − | + |
| BiesA22 (1) | Rps. palustris | 12.0 (0.5) | + | − | +++ |
| HaAB3 (4) | Rmi. vannielii | 13.7 (0.8) | − | − | − |
| HaB4 (1) | Rmi. vannielii | 14.7 (0.6) | − | − | − |
| HaB5 (1) | Rmi. vannielii | 16.3 (0.8) | − | − | − |
| BiesB14 (1) | Rmi. vannielii | 13.7 (0.8) | − | − | − |
| BiesA6 (2) | Rsp. fulvum | 5.3 (0.3) | − | − | − |
| BiesB4 (2) | Rpl. elegans | 27.0 (1.0) | − | − | − |
| BiesB15 (1) | Rpl. elegans | 14.0 (0.5) | − | − | − |
| BiesB16 (1) | Rpl. roseus | 19.2 (1.0) | − | − | − |
a
A single representative of each genotype was tested.
b
Abbreviations: Rps., Rhodopseudomonas; Rmi., Rhodomicrobium; Rsp., Rhodospirillum; Rpl., Rhodoplanes.
c
Doubling time in media containing 2 mM benzoate. The data shown are averages of three measurements. The values in parentheses are standard deviations.
d
+, homology; −, no homology.
e
Utilization of the initial amount (1 mM) of 3-chlorobenzoate in the presence or absence of 1 mM benzoate after 3 weeks, indicated as follows: −, <5%; +, 5 to 25%; +++, >50%.