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Effect of heat treatment at 55°C on viable numbers of wt L. monocytogenes (▴) and AK01 (▪). The viability of both cultures in absence of heat treatment is also represented for the wt (▵) and AK01 (□). Cells were cultivated aerobically at 22°C in BHI broth (pH 7), harvested, and resuspended in ACES buffer (50 mM, pH 7.0) before treatment. Values are means of triplicate measurements from a representative experiment. Bars represent standard deviations (n = 3)
