FIG. 1.

Temporal and spatial colonization of the light transmission chamber by P. fluorescens HK44 visualized by salicylate-inducible bioluminescence. The first panel shows a view of the chamber under room lighting. Drilled sampling port locations are marked by crosses. The superimposed rectangle defines the region of the chamber shown in each of the subsequent panels. The images have been artificially colorized to clearly show differences in emission intensity (arbitrary LU). The cross superimposed on each image indicates the location of the inoculation port, which is also shown as the uppermost port in the first panel. Digital image processing was carried out using Transform 3.4 (Fortner Software LLC), and processed images were prepared for publication using Photoshop 5.0 (Adobe Systems Inc., San Jose, Calif.).