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. 2025 Nov 21;16:1688090. doi: 10.3389/fimmu.2025.1688090

Table 2.

Mixed linear model estimates of peripheral lymphocyte subsets after first ublituximab infusion.

Lymphocyte subset Model F (df)1 p-valuea ICC Baseline Estimated mean (95% CI) Day 180 Estimated mean (95% CI) %Δ Baseline→day 180b Post hoc: significant pairwise differences (mean difference* [95% CI], p-adj)**
CD3+
T cells
6.25
(9, 72.1)
<0.001 0.48 1,639
(1,289–1,990)
1,452
(927–1,976)
−11.6% Baseline vs. day 0: 1,577 [823, 2,331], p < 0.001;
all other pairwise comparisons: NS (p = 1.000)
CD3+CD8+
T cells
8.09
(9, 71.2)
<0.001 0.70 505
(427–583)
450.6
(285–616)
−10.8% Baseline vs. day 0: −406 [−616, −196], p < 0.001;
baseline vs. day 14: −189 [−369, −9], p = 0.03;
baseline vs. day 30+: NS (p = 1.000)
CD4+CD4+
T cells
6.89
(9, 71.9)
<0.001 0.56 1,088
(828–1,348)
956
(584–1,328)
−12.1% Baseline vs. day 0: −1,133
[−1,649.5, −617.2], ≥0.31
all other pairwise comparisons: NS (p ≥0.31)
CD19+ naive
B cells
19.8
(9, 63.9)
<0.001 0.017 231
(200–262)
0.1
(−56 to 56)c
−100% All post-baseline time points (0–180 days) vs. baseline: significant reduction (all p < 0.001);
no differences among post-baseline (all p ≥ 0.99)
CD16+CD56+ NK cells 2.64
(9, 71.7)
0.011 0.49 274
(183–364)
258
(123−392)
−5.5% Baseline vs. day 0: −239 [−432.4, −45.0],
p = 0.004; day 0 vs. day 7: −230.2 [−427.3, −33.1], p = 0.008; all others NS (p ≥ 0.71)

ICC, intraclass correlation coefficient; NS, not significant.

a

Model F (df): F statistic and degrees of freedom from the mixed-effects model.

b

%Δ expresses the relative percentage change in cell counts from baseline (day −3) to day 180. Negative values indicate a decrease; positive values indicate an increase.

c

Negative values in confidence intervals are statistical artifacts resulting from the estimation process and do not represent actual negative cell counts; these should be interpreted as indicating very low or undetectable levels.

*For post hoc pairwise comparisons, the mean difference is reported with its 95% CI and adjusted p-value. The sign of the mean difference indicates the direction of change: negative values mean the comparator group has a lower mean than the reference group, and positive values indicate a higher mean. These values reflect the actual differences between groups as estimated by the statistical model.

**Post hoc: Key significant pairwise differences from post hoc tests, with mean difference, 95% CI, and adjusted p-value.