FIG. 3.
Visualization by CLSM projected z series of the localization of Salmonella serovar Thompson(pWM1007) in the phyllosphere of inoculated cilantro plants. (A) Microcolonies of Salmonella serovar Thompson (GFP) above a vein of a cilantro leaf 2 days after inoculation. (B) High density of Salmonella serovar Thompson (GFP) cells in the vein area of a senescent cilantro leaf 9 days after inoculation. (C) Large aggregate of Salmonella serovar Thompson (GFP) and P. agglomerans (DsRed) cells on the vein of a cilantro leaf 7 days after inoculation. (D) Heterogeneous bacterial aggregate comprised of Salmonella serovar Thompson (GFP), P. agglomerans (DsRed), and natural microflora (visualized with the stain SYTO 62 and assigned the pseudocolor blue) (arrow) on the cilantro phylloplane. Bars, 10 μm.