TABLE 2.
Plot | Sampling date | No. of isolatesa
|
|||||||
---|---|---|---|---|---|---|---|---|---|
Nopaline type
|
Octopine type
|
||||||||
Biovar 1
|
Biovar 2
|
Biovar 1
|
Biovar 2
|
||||||
Soil | Root | Soil | Root | Soil | Root | Soil | Root | ||
Beni Tamou | March 1990 | 16 | NAb | 0 | NA | 8 | NA | 0 | NA |
April 18 1994 | 11 | 16 | 13 | 10 | 0 | 0 | 0 | 0 | |
June 23 1994 | 3 | 28 | 19 | 5 | 0 | 0 | 0 | 0 | |
Boughrara | April 12 1998 | 3 | 3 | 2 | 0 | 0 | 0 | 0 | 0 |
July 6 1998 | 2 | 2 | 6 | 1 | 0 | 3 | 0 | 0 | |
Ouled Mimoun | April 15 1998 | 2 | 6 | 3 | 1 | 0 | 0 | 0 | 0 |
July 7 1998 | 2 | 5 | 5 | 3 | 0 | 2 | 0 | 0 |
The presence of nopaline-type Ti plasmids was determined by utilization of nopaline, synthesis of nopaline in incited tumors, sensitivity to K84, and hybridization with an nos probe. The presence of octopine-type Ti plasmids was determined by utilization of octopine, agropine, and mannopinic acid; synthesis of octopine, agropine, and mannopine in incited tumors; lack of sensitivity to K84; and lack of hybridization with an nos probe. Soil, bulk soil; Root, roots and soil particles tightly adhering to roots of weeds.
NA, not applicable.