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. 2002 Jul;68(7):3251–3260. doi: 10.1128/AEM.68.7.3251-3260.2002

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Copyright © 2002, American Society for Microbiology

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FIG. 6. — (a) SDS-PAGE of GPC-purified SbpA derivatives. Lane 1, rSbpA/Strep-tag I; lane 2, rSbpA_31-1068/Strep-tag I. (b) Dot blot assays indicating the accessibility of Strep-tag I to Strep-Tactin-ALP in the soluble S-layer protein fractions. Spot 1, rSbpA; spot 2, rSbpA/Strep-tag I; spot 3, rSbpA_31-1068/Strep-tag I. (c) Immunoblot analysis for detection of recombinant streptavidin bound by recrystallization products prepared with rSbpA (lane 1), rSbpA/Strep-tag I (lane 2), and rSbpA_31-1068/Strep-tag I (lane 3). Monomers of core streptavidin show a molecular mass of 13,000 Da on SDS gels. In the case of streptavidin, low nonspecific adsorption was observed for the square S-layer lattice formed by rSbpA.