FIG. 7.
Lanes 1 to 4, SDS-PAGE pattern of SDS-extracted whole cells of E. coli HMS174(DE3) containing pET28a with the sbpA derivative encoding rSbpA31-1068/Bet v1 before (lane 1) and 1, 3, and 5 h after (lanes 2 to 4, respectively) induction of S-layer fusion protein expression. Lane 5, rSbpA31-1068/Bet v1 purified by GPC. Lanes 6 and 7, immunoblot analysis using a polyclonal rabbit antiserum raised against SbpA; SDS extracts of the S-layer protein from B. sphaericus CCM 2177 or rSbpA31-1068/Bet v1 were applied. Lanes 8 and 9, immunoblot analysis using the monoclonal antibody BIP1 raised against Bet v1; the samples corresponding to lanes 6 and 7 were applied.