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Electron micrographs of negatively stained preparations. (a) Self-assembly properties of rSbpA. (b) Ability of rSbpA to recrystallize on poly-l-lysine-coated EM grids. (c) Formation of the square S-layer lattice by rSbpA31-1068/Bet v1 on peptidoglycan-containing sacculi of B. sphaericus CCM 2177. (d) Immunogold labeling of recrystallization products obtained with rSbpA31-1068/Bet v1 using the Bet v1-specific monoclonal antibody BIP1. Bars, 200 nm.
