TABLE 2.
Comparison of SLH domain-containing cell-associated exoproteins from gram-positive bacteria
| Species | Strain | Protein | GenBank accession no. | Number, localization, and order of SLH motifs | pI
|
||
|---|---|---|---|---|---|---|---|
| Leader peptide | SLH domaina | Remaining protein sequence | |||||
| Bacillus sphaericus | CCM 2177 | S-layer protein SbpA | AF211170 | 1, 2, 3 (N terminal) | 11.31 | 4.49 | 4.63 |
| P1 | S-layer protein | A45814 | 1, 2, 3 (N terminal) | 11.31 | 4.43 | 4.71 | |
| 2362 | S-layer protein | M28361 | 1, 2, 3 (N terminal) | 11.31 | 4.22 | 5.07 | |
| Bacillus stearothermophilus | PV72/p2 | S-layer protein SbsB | X98095 | 1, 2, 3 (N terminal) | 10.05 | 8.67 | 4.75 |
| Bacillus anthracis | Sterne derivative, substrain 9131 | S-layer protein EA1 | X99724 | 1, 2, 3 (N terminal) | 10.03 | 5.85 | 5.36 |
| S-layer protein SAP | Z36946 | 1, 2, 3 (N terminal) | 10.03 | 5.52 | 6.06 | ||
| Thermoanerobacterium kivui | DSM 2030 | S-layer protein Slp | M31069 | 1, 2, 3 (N terminal) | 10.31 | 6.37 | 4.77 |
| Bacillus brevis | HPD31 | S-layer protein (hexagonal wall protein HWP) | D90050 | 1, 2, 3 (N terminal) | 9.89 | 8.32 | 4.53 |
| 47 | S-layer protein (middle wall protein MWP) | M14238 | 1, 2, 3 (N terminal) | 10.01 | 5.21 | 4.38 | |
| Thermotoga maritima | Outer membran protein Omp-α | X68276 | 1 (N terminal) | 11.05 | 5.75 | 4.56 | |
| Thermus thermophilus | HB8 | S-layer protein SlpA | X57333 | 1 (N terminal) | 11.21 | 5.12 | 4.54 |
| Clostridium thermocellum | NCIMB 10682 | S-layer protein SlpA | U79117 | 1, 2, 3 (N terminal) | 10.31 | 4.21 | 4.36 |
| Cellulosome-anchoring protein | X67506 | 1/2 1, 2, 3, 1/2 1 (C terminal)b | 12.21 | 7.28 | 4.36 | ||
| S-layer protein 1 (cell surface glycoprotein 1) | X67506 | 1/2 1, 2, 3, 1/2 1 (C terminal) | 11.31 | 5.38 | 3.54 | ||
| S-layer protein 2 (cell surface glycoprotein 2) | X67506 | 1/2 1, 2, 3, 1/2 1 (C terminal) | 10.01 | 8.97 | 4.84 | ||
| Exoglucanase XYNX | M67438 | 1, 2, 3 (C terminal) | 10.01 | 4.69 | 4.34 | ||
| Thermoanaerobacterium thermosulfurigenes | EM1 | Endoxylanase | U50952 | 1, 2, 3 (C terminal) | 12.11 | 4.48 | 4.47 |
| Amylopullulanase | M57692 | 1, 2, 3 (C terminal) | 11.21 | 4.67 | 4.30 | ||
| Thermoanaerobacterium saccharolyticum | Endo-1,4-β-xylanase A | M97882 | 1, 2 (C terminal) | 10.89 | 4.41 | 4.36 | |
| Bacillus sp. | KSM-635 | Endoglucanase | M27420 | 1, 2, 3 (N terminal) | 10.31 | 4.87 | 4.02 |
a
pIs of >7 are in boldface (see text).
b
1/2 1, 2, 3, 1/2 1 means that the first SLH motif is missing its N-terminal part, which is appended to the end of the third SLH motif.