FIG. 2.

(A) Western blot analysis of HSP (GroEL, DnaK, DnaJ, ClpC, ClpP, ClpX, and FtsH) production in B. cereus. Cells were grown to mid-exponential phase at 30°C (control) and exposed to mild heat treatment (42°C) for 7.5 and 15 min (m) in the absence and presence of chloramphenicol (cm). (B) Schematic representation of the gene clusters encoding the analyzed HSPs in B. cereus (black arrows) and proteins homologous to heat-induced proteins in B. subtilis (white arrows; HrcA, GrpE, the transcriptional regulator YacB, the redox regulated chaperone YacC, and CtsR), which were not found to be heat induced in B. cereus in our study. For each gene, the percent identity to the B. subtilis homologs and the molecular mass of the encoded protein are indicated. The CIRCE sequence preceding the gene encoding HrcA is indicated by two vertical lines.