TABLE 1.
Spot no. | Proteinb | Groupc | Heated (42°C) for:
|
Stressed by exposure to:
|
|||||
---|---|---|---|---|---|---|---|---|---|
15 mind | 1 h | 4 h | 7°C | NaCl | Ethanol | pH 5 | |||
1 | DnaK | I | >20 | >20 | >20 | 4.2 | >20 | 3 | 2.5 |
2 | ND | I | 7.6 | 7.4 | 8.9 | 20 | 14.9 | 13.7 | |
3 | GroEL | I | 3.5 | 5.5 | 7.5 | 2.1 | |||
4 | YbbT | I | 3.8 | 4.6 | 2.1 | 2.4 | 2.5 | 2.6 | |
5 | AldA | I | 10.9 | 12.9 | 2.2 | ||||
6 | ND | II | 2.6 | 4.8 | 8.6 | 3.7 | |||
7 | MreB | II | 2.3 | 2.5 | 2 | ||||
8 | ND | II | 4 | 2.8 | 3.7 | 3.1 | |||
9 | ND | II | 2.9 | 3 | 3.1 | ||||
10 | FolD | I | 2.8 | 6.3 | 3.1 | ||||
11 | Dra | II | 2 | 4.5 | |||||
12 | SodA | III | 2 | 2 | 2 | 2.3 | 4.8 | ||
13 | ND | III | 2 | 2 | 2.1 | ||||
14 | ClpP | III | 3.5 | 3.9 | 4.1 | 3.6 | |||
15 | ND | II | 2.2 | 2.4 | 2.4 | ||||
16 | ND | II | 2 | ||||||
17 | HSP16.4 | I | 8 | 4.4 | 16.2 | ||||
18 | PpiB | II | 2 | 3 | 2.3 | ||||
19 | YflT | III | 4.1 | 6.1 | 5.3 | ||||
20 | ND | II | 2.4 | 2.9 | |||||
21 | ND | II | 3.9 | 2.9 | |||||
22 | ND | II | 7.1 | ||||||
23 | RsbV | II | >20 | 3.7 | 4 | ||||
24 | SpoVG | II | 2.5 | 3.8 | |||||
25 | TrxA | II | >20 | >20 | |||||
26 | ND | III | 4.9 | 2 | 2 | 5.2 | 3.5 | ||
27 | YloH | III | 8 | 7 | 10.4 | ||||
28 | ND | III | 2.3 | 2.2 | 2 | 2.9 | |||
29 | ND | II | 17.6 | 9.9 | 2.2 | ||||
30 | CspE | II | 4.2 | 3.5 | |||||
31 | CspB | II | 2.9 | 2.2 |
Normalized value in stress gel/normalized value in control gel.
Identified heat-induced protein. ND, N-terminal amino acid sequence not determined.
Grouping is based on time point of induction (see text for details).
Less-than-twofold induction.